Journal: Journal of Clinical Medicine

Article Title: Efficacy of Combining Multiple Electromagnetic Navigation Bronchoscopy Modalities for Diagnosing Lung Nodules

doi: 10.3390/jcm11247341

Figure Lengend Snippet: Demographic and baseline characteristics.

Article Snippet: Another meta-analysis examined the additional advantage of R-EBUS (1.4 mm, 20 MHz, UM S20-17S; Olympus) in combination with ENB (SuperDimension).

Techniques: Standard Deviation

Journal: Journal of Clinical Medicine

Article Title: Efficacy of Combining Multiple Electromagnetic Navigation Bronchoscopy Modalities for Diagnosing Lung Nodules

doi: 10.3390/jcm11247341

Figure Lengend Snippet: Diagnostic results of ENB and R-EBUS-guided TBLB.

Article Snippet: Another meta-analysis examined the additional advantage of R-EBUS (1.4 mm, 20 MHz, UM S20-17S; Olympus) in combination with ENB (SuperDimension).

Techniques: Diagnostic Assay

Journal: Journal of Clinical Medicine

Article Title: Efficacy of Combining Multiple Electromagnetic Navigation Bronchoscopy Modalities for Diagnosing Lung Nodules

doi: 10.3390/jcm11247341

Figure Lengend Snippet: Performance of ENB and R-EBUS-guided TBLB. Diagnostic accuracy was boosted after adding another modality. NPV, negative predictive value; TBLB1, ENB-guided lung biopsy only; TBLB2, ENB-guided lung biopsy plus needle aspiration; TBLB3, ENB-guided lung biopsy and aspiration plus R-EBUS-guided lung biopsy.

Article Snippet: Another meta-analysis examined the additional advantage of R-EBUS (1.4 mm, 20 MHz, UM S20-17S; Olympus) in combination with ENB (SuperDimension).

Techniques: Diagnostic Assay

Journal: Nature Communications

Article Title: Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice

doi: 10.1038/s41467-021-27860-x

Figure Lengend Snippet: a , b Transferrin (Tf) trafficking assay showing the decrease of Tf fluorescence in AMΦ (WT and Trpml3 − / − ) within 20 min after the pulse with Tf-AlexaFluor488 (Tf accumulation). Mean ± SEM, 4 biologically independent experiments, each. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; Two-way ANOVA followed by Bonferroni’s post hoc test. c Tf fluorescence in AMΦ (WT and Trpml3 − / − ) after 20 min pulse with Tf-AlexaFluor488 (0 min timepoint, measures Tf uptake). Mean ± SEM, four biologically independent experiments. * p < 0.05, ** p < 0.01, Student’s t test, unpaired, two-tailed. d Representative confocal images and quantification of the colocalization of EEA1 and Tf in AMΦ (WT and Trpml3 − / − ). Statistical analysis was performed using Student’s t test, unpaired, two-tailed. Mean ± SEM, three biologically independent experiments. * p < 0.05, *** p < 0.001. e , f TfR expression analysis using Western blot. e Shown are two independent WB blots for TfR (90 kDa) and ß-Actin (45 kDa; loading control) using 5 WT and 5 Trpml3 − / − AMΦ lysates on each blot. f Quantification of WB data as shown in e . TfR protein was normalized to ß-Actin and values from Trpml3 − / − AMΦ were normalized to WT AMΦ. One single dot corresponds to one mouse, each (mean ± SEM). g , h Whole-cell patch-clamp experiments to determine membrane capacitance (measure of cell surface area). GTPγS induces an increase in surface area. Co-application of ML3-SA1 significantly reduces the effect of GTPγS in WT AMΦ, but not in Trpml3 − / − AMΦ ( = loss of membrane surface). Significance: GTPγS vs. GTPγS + ML3-SA1 from 140 to 150 sec *, from 152 to 156 sec **, from 158 to 172 sec ***, from 174 to 194 **, then till 200 sec *** (yellow dots). * p < 0.05, ** p < 0.01, *** p < 0.001; two-way ANOVA followed by Tukey’s multiple comparisons test. Shown are mean values ± SEM, n (in parentheses) = biologically independent experiments. i , j Bar diagrams (mean ± SEM) showing the parameters Tau (= time until 2/3 of the maximum amplitude is reached) and Delay (= time until capacitance changes). * p < 0.05, Student’s t test, unpaired, two-tailed. Source data are provided as a Source Data file.

Article Snippet: Recycling kinetics were analysed by chasing for 5, 10, 15, and 20 min in complete media plus 20 µg/ml unconjugated transferrin (T0665, Sigma).

Techniques: Fluorescence, Two Tailed Test, Expressing, Western Blot, Patch Clamp